ABSTRACT
The quorum sensing [QS] of pathogens has been found to affect their biofilm forming ability, making it a potential target for anti-microbial therapy. The present research aimed to evaluate the anti-QS activities of different extracts and isolated phytochemicals from Liriodendron hybrid barks and their roles in the inhibition of the growth and biofilm formation of methicillin-resistant Staphylococcus aureus [MRSA]. The assays on the inhibition of QS by the five extracts [n-hexane, dichloromethane, ethyl acetate, acetone, and methanol] and eight isolated compounds were carried out by using both the indicator strains Chromobacrerium violaceum CV026 and C. violaceum ATCC12472. The in vitroeffects of the five extracts and eight isolated compounds on MRSA biofilm were also preliminarily evaluated using crystal violet micro titer plate assays. The results suggested that the dichloromethane extract showed anti-QS and MRSA biofilm inhibitory activities and the n-hexane extract possessed only MRSA biofilm inhibitory effect. The dichloromethane extract could serve as a source for developing bacterial intervention strategies targeting microbial QS system. All eight isolated compounds showed no anti-QS and biofilm formation inhibiting activities. So further researches are still being required to purify and identify the compounds possessing anti-QS and biofilm inhibitory effects from the dichloromethane and n-hexane extracts
ABSTRACT
Objective To study the phenolic compounds from Sagina japonica. Methods The separation and purification were carried out by silica gel Sephadex LH-20 gel and RP C-18 colomn chromatography. The structures were identified by spectra. Results Nine phenolic compounds were isolated from S. japonica. They were identified as:p-E-methoxy cinnamic acid methyl ester (Ⅰ),umbelliferone (Ⅱ),7-methoxy coumarin (Ⅲ),5,7-di-hydroxy coumarin (Ⅳ),5,7-di-methoxy coumarin (Ⅴ),cerarvensin-7-O-glucoside (Ⅵ),5,7,2'-trihydroxy-8-methoxy flavone (Ⅶ),5,7-di-hydroxy-8,2'-di-methoxy flavone (Ⅷ),5,7,3',4'-tetrahydroxy-6-methoxy flavone (Ⅸ). Conclusion All of these compounds are first isolated from S. japonica.